On the basis of the investigation of the content and distribution of heavy metals in sediments and benthic organisms from the sewage stream in guangzhou city , the antagonistic effect of selenium on the harm of mercury ( hg2 + ) to the tissues in swordtail fish ( xiphophorus helleri ) , the 22nd offspring purely cultivated by pearl river fishery research institute , were measured by physiological and biochemical indexes and observation of electron microscopy 本研究中首先調(diào)查了廣州市河涌沉積物及底棲生物體內(nèi)重金屬含量及分布,并以珠江水產(chǎn)研究所培育的水生實(shí)驗(yàn)動(dòng)物?劍尾魚為實(shí)驗(yàn)材料,利用環(huán)境生物技術(shù),電鏡觀察等技術(shù)方法研究了重金屬?汞對(duì)劍尾魚組織的毒害及機(jī)體必需微量元素硒對(duì)其毒害的拮抗性影響。
The main study in this paper included as follows : the content and distribution of heavy metals in sediments and benthic organisms from the sewage stream in guangzhou city ; the acute toxicity and joint toxicity of mercury and selenium to swordtail fish ( xiphophorus helleri ) ; the damage of mercury to the indexes of antioxidant system in the gills and livers in swordtail ( including the measurement of the activities of total antioxidative capacity [ t ~ aoc ] , superoxide dismutase [ sod ] , glutathione peroxidase [ gsh - px ] and the concentration of malondiald - ehyde [ mda ] ) and the relief effects of selenium on it , as well as the physiological damage of mercury on the tissues , namely : the antagonistic effect of na + - k + ~ atpase activity on the tissues between selenite and mercury , and the ultrastructural damage under the exposure of mercury 研究?jī)?nèi)容主要有:廣州市河涌沉積物及底棲生物體內(nèi)重金屬含量及評(píng)價(jià);汞和硒對(duì)劍尾魚的急性毒性和聯(lián)合毒性及安全濃度的評(píng)價(jià);汞對(duì)劍尾魚鰓和肝臟中抗氧化系統(tǒng)的毒性,包括對(duì)總抗氧化能力、超氧化物歧化酶、谷胱甘肽過(guò)氧化物酶活力及丙二醛含量的測(cè)定及硒對(duì)其保護(hù)作用;汞對(duì)劍尾魚組織生理毒性即:汞對(duì)na ~ + - k ~ + atpase活力的影響及硒的保護(hù)作用和汞和對(duì)劍尾魚組織超微結(jié)構(gòu)的損傷等。以高氯酸?硝酸消化法和火焰原子吸收分光光度法測(cè)定了廣州市河涌沉積物和底棲生物中重金屬含量。